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KMID : 0374019960190030551
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Ewha Medical Journal
1996 Volume.19 No. 3 p.551 ~ p.556
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The Effects of Vero Cell-Conditioned Medium on Mouse Late 2-Cell Embryo
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Abstract
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This study was conducted to evaluate the ability of Vero cell-conditioned medium for
supporting mouse embryo development in vitro.
The mouse late 2-cell embryos were cultured in control media(Ham's F-10+10%FBS), media
with Vero cell monolayer and Vero cell-conditioned media for 4 days and measured the
hatching rate and cell number in the blastocyst stage.
The hatching rate in experimental groups was increased significantly compared with embryos
in control group(p<0.01). On the other hand, the degree of blastomere fragmentation exhibited
a opposite trend to that of that developmental capacity(p<0.05). And also the cell numbers of
expanded blastocysts in experimental groups were increased significantly compared with the
control group(p<0.001). There was, however, no difference between experimental groups.
These results indicate that Vero cell-conditioned medium supported the mouse embryo
developments as a Vero cell monolayer. And the mechanism for enhancement of the
development potential of embryos may be releasing the embryotrophic factor during the
medium-conditioning period.
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